CBS Imaging Core Facility

 

Overview

The microscope systems in the imaging facility are made freely available to members of CBS , MCB and other departments. Reserve time on the systems using the ONLINE SCHEDULER. If you would like to become a registered user, please contact Steve Turney  (sturney [at] mcb [dot] harvard [dot] edu).

The imaging facility comprises seven microscope systems: three Zeiss upright confocal microscope systems (Pascal, LSM-510 Meta, LSM-710), two Olympus upright confocal microscope systems (FV1000 and FV1000MPE), a  Zeiss upright fluorescence microscope with an ApoTome attachment, a Nikon inverted microscope system equipped with TIRF optics and an incubation chamber for time lapse imaging of cell cultures, and a custom-built resonant-galvo multiphoton microscope system. All of the confocal microscope systems have motorized stages and software to allow automatic tiled image acquisition. The confocal systems are primarily used for epifluorescence imaging and are not set up well for transmitted light imaging (only the Zeiss Pascal system has a CCD camera and DIC optics). The Olympus FV1000MPE and Zeiss LSM-510 systems are set up for live imaging.

 

Microscope systems

 

Summary of features and applications

  Slides Live Imaging 2 Photon TIRF Motorized Stage CCD Camera TL** Optics Spectral Imaging
Nikon N-STORM* + +   + + + +  
Nikon TIRF* + +   + + + +  
Zeiss 710 +       +   + +
Zeiss 510 + +     +     +
Zeiss Pascal +       + + +  
Zeiss ApoTome +         + +  
Olympus FVB + + +   +   +  
Olympus FVA +       +   + +
Video-rate multiphoton   + +          

* Inverted microscope stand (all other systems have upright stands)
**Transmitted Light

 

Available laser lines on confocal/multiphoton systems

  405nm 440nm 458nm 488nm 514nm 543nm 561nm 594nm 633nm 2 Photon
Nikon N-STORM +   +       +   +  
Zeiss 710 + + + + +   + + +  
Zeiss 510     + + + +     +  
Zeiss Pascal   * + + + +        
Olympus FVB ** + + + +   +   + +
Olympus FVA   + + + +   +   +  
Video-rate multiphoton                   +

* 436nm laser line
** On secondary scanhead for stimulation and photoconversion