This microscope is set up for time-lapse multi-channel imaging of cell cultures. It is fully motorized and has a Tucsen Dhyana 95 sCMOS camera, a Lumencor Sola Light Engine (solid-state white light source), and filter cubes for CFP, GFP, YFP, mCherry, and NADH. Available objectives include: 10x, 20x, and 40x phase-contrast, 60x water-immersion, 40x and 60x oil-immersion, and 100x TIRF. Micro-Manager is used for image acquisition.
The system is equipped with an Air-Therm heater that circulates and warms the air in the environmental chamber.
Examples
- Long term time lapse imaging of cell cultures
- TIRF imaging
- Combined fluorescence and transmitted-light imaging
Advantages
- Nikon Perfect Focus 1.0 (autofocus)
- Incubation chamber with controlled temperature
- Motorized stage allows time lapse imaging of multiple areas
Disadvantages
- No optical sectioning except TIRF