Nikon N-STORM super-resolution microscope (Ti-E stand)

This system is set up for TIRF imaging and for 2D or 3D super-resolution single-molecule localization using either STORM imaging or Double-Helix PSF imaging, respectively. The Ti-E inverted microscope is equipped with 40x, 60x, and 100x Apo TIRF objectives. Available laser lines are 405nm, 488nm, 561nm, and 647nm. The system has a Hamamatsu ORCA-Flash 4.0 sCMOS camera for TIRF imaging and an Andor iXon Ultra DU897 EM-CCD camera for DH-PSF imaging. NIS-Elements is used for image acquisition.

Examples

1. Tracking directed motion of single molecules
2. Imaging cellular structures with sub-diffraction limit resolution
3. TIRF imaging

Advantages

1. Nikon Perfect Focus (autofocus)
2. Incubation system for long-term time-lapse imaging of cell cultures

Note: To sign up, please first contact Steve Turney (sturney@mcb.harvard.edu) for training.