Zeiss LSM 5 Pascal confocal microscope system (Axiovert 200M stand)

This system has 436nm, 488nm, 514nm and 543nm laser lines. The confocal system has two fluorescence channels and a transmitted light detector. The microscope also has an AxioCam HRc (color CCD) camera. Available objectives include: 10x and 20x air; 40x and 63x water-immersion; and 25x, 40x, 63x, and 100x oil-immersion.

Examples

1. Imaging of CFP, YFP and RFP
2. Tiled image acquisition using CCD camera or confocal

Advantages

1. Microscope is fully motorized
2. Zen software

Disadvantages

1. No spectral channels
2. No 633nm laser line for confocal imaging of alexa-647 or cy5