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Zeiss LSM-710 confocal microscope system (Axio Imager.Z1 stand)

This microscope system has 405nm, 440nm, 488nm, 514nm, 561nm, 594nm and 633nm laser lines.

The primary dichroics are suitable for simultaneous excitation of CFP and YFP. The system will have two spectral channels and a 32-channel quasar detector (for lambda scans).

The stage is motorized to allow automated tiled image acquisition.

Examples

  1. Imaging of CFP, YFP and mCherry in fixed tissue
  2. Acquisition of spectral emission profile in single scan

Advantages

  1. All spectral channels (no barrier filters)
  2. High detection sensitivity

Disadvantages

  1. No multiphoton laser
  2. No DIC optics