A high-throughput approach to enable the reconstruction of connectomes using light microscopy requires further technical innovation. To achieve this goal, five groups from Harvard, Stanford, and MIT are combining several new methods that they have developed over the past few years, viz.:
2) automated high-throughput tissue preparation, imaging, and analysis techniques (Jeff Lichtman, Harvard);
3) super-resolution fluorescence microscopy (Xiaowei Zhuang, Harvard);
4) methods for molecular screening of synaptic neuropil (Stephen Smith, Stanford); and
5) computational approaches to neural circuit tracing (Sebastian Seung, MIT).