Combined voltage imaging and optogenetics reveal behavior dependent changes in hippocampal dynamics


February 27, 2019 - 12:00pm
Northwest Building, Room 243
About the Speaker
Yoav Adam
Speaker Title: 
Postdoctoral Fellow
Speaker Affiliation: 
Cohen Lab

A technology to record membrane potential from multiple neurons, simultaneously, in behaving animals will have a transformative impact on neuroscience research. Genetically encoded voltage indicators are a promising tool for these purposes, but were so far limited to single-cell recordings with marginal signal to noise ratio (SNR) in vivo. We developed improved near infrared voltage indicators, high speed microscopes and targeted gene expression schemes which enabled recordings of supra- and subthreshold voltage dynamics from multiple neurons simultaneously in mouse hippocampus, in vivo. The reporters revealed sub-cellular details of back-propagating action potentials and correlations in sub-threshold voltage between multiple cells. In combination with optogenetic stimulation, the reporters revealed brain state-dependent changes in neuronal excitability, reflecting the interplay of excitatory and inhibitory synaptic inputs. These tools open the possibility for detailed explorations of network dynamics in the context of behavior.